Types of containers for egg collection. (A) Plastic cup lined with filter paper used for individual females. The cup is filled with enough H₂O to ensure the filter paper remains wet. Mesh at the top of the container allows the female to have access to cotton wetted with sugar H₂O if necessary. (B,C) To collect eggs en masse, a cup with H₂O and lined with filter paper can be placed in a cage with gravid and mated female mosquitoes or (D,E) a filter paper can be placed on top of sponges soaked in H₂O contained in a Petri dish. (F–I) Alternatively, a 9-cm-diameter round filter paper can be cut from the edge to the center to create a funnel. The funnel is then placed on top of a cup filled with H₂O touching the paper on which females will land and oviposit.