Recipe

Phosphate-Buffered Saline (PBS; pH 7.4)

8 mm Na2HPO4 (Millipore-Sigma S9763-100G)

1.5 mm KH2PO4 (Millipore-Sigma P5655-100G)

3 mm KCl (Millipore-Sigma P9541-500G)

137 mm NaCl (Millipore-Sigma S3014-5KG)

Adjust pH to 7.4 using HCl and autoclave. Store the solution indefinitely at room temperature. If any precipitate forms in the buffer during storage, it should be redissolved by warming up the buffer before use.

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  1. doi:10.1101/pdb.rec108350 Cold Spring Harb Protoc 2024: pdb.rec108350- © 2024 Cold Spring Harbor Laboratory Press
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