PBT Buffer (pH 7.4)
Phosphate-buffered saline (PBS; pH 7.4)
0.5% (w/v) Bovine serum albumin (BSA) (Millipore-Sigma A7030-500G)
0.05% (v/v) Tween-20 (Millipore-Sigma P1379-250ML)
Filter-sterilize. The solution can be stored for 1 mo at 4°C. However, to avoid any source of contamination, we recommend preparing it immediately before use.
