Recipe

Mosquito Antibody Dilution Buffer (MADB)

Reagent Final concentration Amount
Goat serum 10% (v/v) 50 mL
Bovine serum albumin 3% (w/v) 15 g
Triton-X 0.05% (v/v) 250 µL
Phosphate-buffered saline 90% (v/v) 450 mL

  • This recipe makes a 500-mL solution that can be stored as 50-mL aliquots for up to 6 mo at −20°C.

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  1. doi:10.1101/pdb.rec108461 Cold Spring Harb Protoc 2024: pdb.rec108461- © 2024 Cold Spring Harbor Laboratory Press
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