Drosophila Artificial Hemolymph
| Reagent | Concentration | Molecular weight | Amount per 1000 mL |
|---|---|---|---|
| NaCl | 103 mm | 58.44 g/mol | 6.0193 g |
| KCl | 3 mm | 74.55 g/mol | 0.2237 g |
| HEPES | 5 mm | 238.3 g/mol | 1.1915 g |
| CaCl2 | 1.5 mm | 110.99 g/mol | 0.1665 g |
| MgCl2•6H2O | 4 mm | 203.3 g/mol | 0.8132 g |
| NaHCO3 | 26 mm | 84 g/mol | 2.1840 g |
| NaH2PO4•H2O | 1 mm | 137.99 g/mol | 0.1380 g |
| Trehalose | 10 mm | 378.33 g/mol | 3.7833 g |
| Sucrose | 7 mm | 342.3 g/mol | 2.3961 g |
| Glucose | 10 mm | 180.16 g/mol | 1.8016 g |
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Adjust to pH 7.2 with NaOH. The solution can be stored for ∼1 wk at 4°C without sterilization.
