Recipe

High-K+ (100 mm) Saline for Neuronal Stimulation

Concentration Molecular weight Amount per 250 mL
NaCl 6 mm 58.44 g/mol 0.0877 g
KCl 100 mm 74.55 g/mol 1.8638 g
HEPES 5 mm 238.3 g/mol 0.2979 g
CaCl2 1.5 mm 110.99 g/mol 0.0416 g
MgCl2•6H2O 4 mm 203.3 g/mol 0.2033 g
NaHCO3 26 mm 84 g/mol 0.5460 g
NaH2PO4•H2O 1 mm 137.99 g/mol 0.0345 g
Trehalose 10 mm 378.33 g/mol 0.9458 g
Sucrose 7 mm 342.3 g/mol 0.5990 g
Glucose 10 mm 180.16 g/mol 0.4504 g

  • Adjust to pH 7.2 with NaOH. The solution can be stored for ∼1 wk at 4°C without sterilization. It can be diluted to lower concentrations (e.g., 10 or 30 mm KCl) with Drosophila artificial hemolymph, if desired.

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  1. doi:10.1101/pdb.rec108346 Cold Spring Harb Protoc 2024: pdb.rec108346- © 2024 Cold Spring Harbor Laboratory Press
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