Recipe

Proximity Ligation Assay Wash Buffer B (Lab-Made)

Prepare a 1× stock solution containing:

200 mm Tris, pH 7.5

100 mm NaCl

Add H2O to give a final volume of 1 L

Autoclave the solution for 45 min at 121°C with a slow vent or filter-sterilize. Store indefinitely at room temperature.

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  1. doi:10.1101/pdb.rec108374 Cold Spring Harb Protoc 2024: pdb.rec108374- © 2024 Cold Spring Harbor Laboratory Press

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