Fixative Solution for Mosquito Tissues
| Reagent | Amount | Final concentration |
|---|---|---|
| 20× Paraformaldehyde aqueous solution (PFA) (EMS 15713-S) | 10 mL | 4% |
| 0.2 m Millonig's phosphate buffer (EMS 11582-05) | 25 mL | 0.1 m |
| 10% Triton X-100 (Sigma-Aldrich 93443 diluted 1/10 in H2O) | 1.25 mL | 0.25% |
| Double-distilled H2O | 13.75 mL |
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Makes 50 mL. Store for up to 2 wk at 4°C.
