Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples
- ↵1Correspondence: pc72{at}duke.edu
Abstract
Chromatin immunoprecipitation (ChIP) is a common approach for studying the binding pattern of proteins on DNA sequences or the landscape of histones with different marks throughout the genome. ChIP is used on various organisms, including Drosophila. This protocol provides a detailed overview of the immunoprecipitation portion of a ChIP procedure from samples of Drosophila nervous systems, specifically antennae and brains, that have already been fixed and sheared. These methods can be applied to other tissues of interest after optimizing for sample size and other relevant parameters.
Footnotes
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From the Drosophila Neurobiology collection, edited by Bing Zhang, Ellie Heckscher, Alex C. Keene, and Scott Waddell.
