Wash Buffer II for ChIP (High-Salt)
| Reagent | Stock solution | Manufacturer information | Volume | Final concentration |
|---|---|---|---|---|
| K-HEPES | 1 m, pH 7.6 | Boston BioProducts BBH-76-K | 500 μL | 50 mm |
| NaCl | 5 m | Invitrogen AM9760G | 1 mL | 500 mm |
| EDTA | 500 mm, pH 8.0 | Invitrogen 15575020 | 20 μL | 1 mm |
| EGTA | 500 mm, pH 8.0 | VWR J60767 | 20 μL | 1 mm |
| Triton X-100 | 10% (v/v) | Sigma-Aldrich T9284 | 1 mL | 1% (v/v) |
| Na-deoxycholate | 10% (w/v) | bioPLUS 40430018 | 100 μL | 0.1% (w/v) |
| SDS | 10% (w/v) | Invitrogen 15553027 | 100 μL | 0.1% (w/v) |
| Nuclease-free H2O | 7260 μL |
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Makes a total of 10 mL. Store for up to 2 wk at 4°C.
