YTCK Liquid Medium
Mix 16 g of tryptone, 10 g of yeast extract, and 5 g of NaCl. Add H2O and mix to dissolve all reagents. Adjust pH to 7.0 with NaOH. Adjust volume to 1 L with deionized H2O. Sterilize by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle. Allow the medium to cool down to 60°C. Immediately before use, add 1 mL of a sterile carbenicillin stock solution, for a final carbenicillin concentration of 100 µg/mL, and 1 mL of kanamycin stock solution, for a final kanamycin concentration of 50 µg/mL. YTCK liquid medium should always be prepared fresh.
