Recipe

Baines Solution

Prepare an aqueous solution containing 135 mm NaCl, 5 mm KCl, 2 mm CaCl2, 4 mm MgCl2, 5 mm TES buffer (Fisher AC207510250), and 36 mm sucrose. Start with sterile solutions and water, or filter-sterilize the final mixture. Store for up to 6 mo at 4oC.

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  1. doi:10.1101/pdb.rec108589 Cold Spring Harb Protoc 2025: pdb.rec108589- © 2025 Cold Spring Harbor Laboratory Press

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