Table 2.

An overview of minimalist libraries based on the 4D5 framework

Library	Valency	Frame work	Diversification scheme				Observed maximum K_D (nM)	Reference(s)
Library	Valency	Frame work	L3	H1	H2	H3	Observed maximum K_D (nM)	Reference(s)
Lib-scFv	Mono	4D5	—	Degenerate	Degenerate	DVK	100	Sidhu et al. 2004
Lib-scFv₂	Bi		—	Degenerate	Degenerate	DVK	>100	Sidhu et al. 2004
Lib 1-Fab	Mono		—	Degenerate	Degenerate	DVK	200	Lee et al. 2004b
Lib 1-Fab’-zip	Bi		—	Degenerate	Degenerate	DVK	41	Lee et al. 2004b
Lib 2-Fab	Mono		—	Degenerate	Degenerate	DVK	0.6	Lee et al. 2004a
Lib 2-Fab	Mono		—	Degenerate	Degenerate	NVT	NA	Lee et al. 2004a
Lib 2-Fab	Mono		—	Degenerate	Degenerate	NNS	NA	Lee et al. 2004a
Fab'-zip	Bi		—	Degenerate tetranomial	Degenerate tetranomial	Degenerate tetranomial^a	>10,000	Fellouse et al. 2004
Library A	Bi		—	YS	YS	YS^a	>200	Fellouse et al. 2005
Library B	Bi		YS	YS	YS	YS^a	60	Fellouse et al. 2005, 2006
Library C	Bi		YS^a^,^b	YS^b	YS^b	Degenerate tetranomial^a,b	1.6	Fellouse et al. 2005, 2006
Library D	Bi		Tailored Y/S/G + 19/20^a,b	YS^b	YS^b	Tailored Y/S/G + 19/20^a,b	4.4	Fellouse et al. 2007
YS	Bi		YS	YS	YS	YS^a	ND	Birtalan et al. 2008
Y/S/R	Bi		YS	YS	YS	Trinomial Y/S/R^a	3.1
Y/S/G	Bi		YS	YS	YS	Trinomial Y/S/G^a	0.3
Y/S/G/R	Bi		YS	YS	YS	Tetranomial Y/S/G/R^a	4.9
Library F	Bi		Tailored Y/S/G + 19/20^a,b	YS^b	YS^b	Tailored Y/S/G + 19/20^a,b	0.7	Persson et al. 2013