Generation of a Phage Display Chicken Single-Chain Variable Fragment Library
- 1Cancer Research Institute, Seoul National University College of Medicine, Seoul 03080, South Korea
- 2Convergence Medicine Research Center, Asan Medical Center, Seoul 05505, South Korea
- 3Bio-MAX Institute, Seoul National University, Seoul 08826, South Korea
- ↵4Correspondence: jjhchung{at}snu.ac.kr
Abstract
Phage-displayed antibody fragment libraries can be constructed using essentially any species that is easily immunized, as long as the immunoglobulin variable region gene sequences are known. This protocol describes the procedures for the generation of a phage-displayed chicken single-chain variable fragment (scFv) library after immunization with a target antigen. Briefly, the rearranged heavy chain variable region (VH) genes and the λ light chain variable region (Vλ) genes are amplified separately and are linked through two separate PCR steps to give the final scFv genes. The genes are then cloned into pComb3XSS to generate the phage display chicken scFv library, which can then be used for test and final library ligations.
Footnotes
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From the Advances in Phage Display collection, edited by Gregg J. Silverman, Christoph Rader, and Sachdev S. Sidhu
