Preparation of VCSM13 Helper Phage for Display Library Reamplification and Bio-Panning
- 1Cancer Research Institute, Seoul National University College of Medicine, Seoul 03080, South Korea
- 2Convergence Medicine Research Center, Asan Medical Center, Seoul 05505, South Korea
- 3Bio-MAX Institute, Seoul National University, Seoul 08826, South Korea
- ↵4Correspondence: jjhchung{at}snu.ac.kr
Abstract
Phage-displayed antibody libraries can be constructed using any species that is easily immunized. The pComb3XSS phagemid vector is commonly used for library cloning and phage display. This phagemid encodes the origin of replication of the filamentous bacteriophage f1 but lacks all the genes required for replication and assembly of phage particles. The replication and the assembly of phage from these phagemids thus requires a “helper” phage that provides the genes essential for those steps during library production and bio-panning. One of those helper phages is VCSM13. In this protocol, we describe the preparation of VCSM13 helper phage. Users should prepare VCSM13 helper phage for library reamplification and for bio-panning.
Footnotes
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From the Advances in Phage Display collection, edited by Gregg J. Silverman, Christoph Rader, and Sachdev S. Sidhu
