LB Plates
Dissolve 25 g of LB (Lysogeny Broth) medium powder (MilliporeSigma L3522) in 1 L of highly pure water with 15 g of Bacto Agar (BD 214010). Autoclave for 15 min at 121°C and 15 psi. When cooled to ∼50°C, pour into 10-cm sterile Petri dishes (20–25 mL each), remove bubbles by flaming the plates with a Bunsen burner, and allow to solidify at room temperature.
Store for up to 3 mo at 4°C.
