LB + 30 µg/mL Kanamycin Plates
Dissolve 25 g of LB (Lysogeny Broth) medium powder (MilliporeSigma L3522) in 1 L of highly pure water (e.g., that produced with a Hydro PicoPure UV Plus system) with 15 g of Bacto Agar (BD 214010). Autoclave for 15 min at 121°C and 15 psi. When cooled to ∼50°C, add 600 µL of 50 µg/µL kanamycin. Pour into 10-cm sterile Petri dishes (20–25 mL each), remove bubbles by flaming the plates with a Bunsen burner, and allow to solidify at room temperature. Store for up to 3 mo at 4°C. Warm to 37°C in an incubator before plating.
