Protocol

Solution-Phase Exome Capture

  1. W. Richard McCombie

Abstract

This protocol describes the construction of a paired-end library of genomic DNA (gDNA) and subsequent capture of specific regions of a genome using NimbleGen sequence capture probes and Illumina TruSeq oligos. The captured DNA, purified and quantitated, is appropriate for use as template in Illumina sequencing systems. A procedure is also provided for magnetic AMPure bead cleanup.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

This article has not yet been cited by other articles.

No Related Web Pages

This Article

  1. Published in Advance November 1, 2016, doi: 10.1101/pdb.prot094680 Cold Spring Harb Protoc © 2017 Cold Spring Harbor Laboratory Press
  1. » Abstract
  2. Full Text (PDF)
  3. All Versions of this Article:
    1. pdb.prot094680v1
    2. 2017/8/pdb.prot094680 most recent

Article Category

  1. Protocol

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Related Content

  1. Related Web Pages

Share

Current Issue

  1. April 2026, 2026 (4)
  1. Current Issue