Protocol

Agarose Gel Size Selection for DNA Sequencing Libraries

  1. W. Richard McCombie

Abstract

Agarose gel electrophoresis may be used to purify fragmented genomic DNA after ligation of adaptors. After electrophoresis, the region of the gel containing the desired size range of DNA is excised, and the DNA is subsequently extracted from the gel and purified by passage through a spin column.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. Published in Advance November 1, 2016, doi: 10.1101/pdb.prot094698 Cold Spring Harb Protoc © 2017 Cold Spring Harbor Laboratory Press
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