Protocol

Library Quantification: Fluorometric Quantitation of Double-Stranded or Single-Stranded DNA Samples Using the Qubit System

  1. W. Richard McCombie

Abstract

Qubit is an accurate and highly sensitive fluorescence-based quantitation system. Several assay kits have been optimized for the Qubit fluorometer, but also function efficiently with other fluorometers. The high-sensitivity dsDNA Qubit Kit has a detection range of 0.2–100 ng. The ssDNA Kit has a detection range of 1–200 ng.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. Published in Advance November 1, 2016, doi: 10.1101/pdb.prot094730 Cold Spring Harb Protoc © 2017 Cold Spring Harbor Laboratory Press
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