Illumina Sequencing of Bisulfite-Converted DNA Libraries
Abstract
Here we describe a standard MethylC-seq protocol using single-read sequencing on an Illumina Genome Analyzer II platform. The protocol involves ligation of methylated sequencing adaptors to sonicated genomic DNA, gel purification, sodium bisulfite conversion, polymerase chain reaction (PCR) amplification, and sequencing.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
- © 2017 Cold Spring Harbor Laboratory Press
