Protocol

Induction of Hyphal Growth in Schizosaccharomyces japonicus

  1. Hironori Niki1
  1. Microbial Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan
  1. ↵1Correspondence: hniki{at}nig.ac.jp

Abstract

Schizosaccharomyces japonicus is a dimorphic yeast. Depending on the nutrient conditions, the transition between growth as yeast cells and growth as hyphal cells can be reversibly induced. In addition to nutrient stress, induced DNA lesions, such as those produced by camptothecin, also induce hyphal growth regardless of the nutrient status. This protocol describes both methods of induction.

Footnotes

  • From the Fission Yeast collection, edited by Iain M. Hagan, Antony M. Carr, Agnes Grallert, and Paul Nurse.

This Article

  1. Published in Advance July 21, 2017, doi: 10.1101/pdb.prot091868 Cold Spring Harb Protoc © 2017 Cold Spring Harbor Laboratory Press
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