DNA Preparation from Schizosaccharomyces pombe
- Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California 94518
- ↵1Correspondence: roguev{at}gmail.com
Abstract
This protocol includes two methods for genomic DNA preparation from Schizosaccharomyces pombe. The first is a quick method for preparation of DNA suitable for polymerase chain reaction (PCR) genotyping. The second, longer method yields high-quality DNA that can be used for amplification of targeting cassettes.
Footnotes
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From the Fission Yeast collection, edited by Iain M. Hagan, Antony M. Carr, Agnes Grallert, and Paul Nurse.
