Topic Introduction

Live Cell Imaging in Fission Yeast

  1. Daniel P. Mulvihill1
  1. School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, United Kingdom
  1. ↵1Correspondence: D.P.Mulvihill{at}kent.ac.uk

Abstract

Live cell imaging complements the array of biochemical and molecular genetic approaches to provide a comprehensive insight into functional dependencies and molecular interactions in fission yeast. Fluorescent proteins and vital dyes reveal dynamic changes in the spatial distribution of organelles and the proteome and how each alters in response to changes in environmental and genetic composition. This introduction discusses key issues and basic image analysis for live cell imaging of fission yeast.

Footnotes

  • From the Fission Yeast collection, edited by Iain M. Hagan, Antony M. Carr, Agnes Grallert, and Paul Nurse.

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This Article

  1. Published in Advance July 21, 2017, doi: 10.1101/pdb.top090621 Cold Spring Harb Protoc © 2017 Cold Spring Harbor Laboratory Press
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