Whole-Mount Immunocytochemistry in Xenopus
- Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, Colorado 80309
- ↵1Correspondence: klym{at}colorado.edu
Abstract
To visualize the effects of experimental perturbations on normal cellular behavior, morphology, and intracellular organization, we use a simple whole-mount immunocytochemical method with Xenopus oocytes, explants, or embryos. This method is applicable to a wide range of systems, including human-induced pluripotent stem cell-derived organoids, and can be used with both chromogenic (horseradish peroxidase/diaminobenzidine) and fluorescent imaging.
Footnotes
-
From the Xenopus collection, edited by Hazel L. Sive.
