Protocol

Einsteck Transplants

  1. Hélène Cousin1
  1. Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003
  1. ↵1Correspondence: hcousin{at}vasci.umass.edu

Abstract

Einsteck procedure refers to a method whereby the experimenter inserts material into the blastocoel cavity of an early amphibian embryo. This procedure is simpler to perform than other types of grafts, such as Spemann–Mangold, and with practice yields a sizable amount of data suitable for statistical analysis. This protocol for Einsteck transplantation in Xenopus describes the insertion of the gastrula-stage blastopore lip into the blastocoel cavity of a host embryo.

Footnotes

  • From the Xenopus collection, edited by Hazel L. Sive.

This Article

  1. Published in Advance January 10, 2018, doi: 10.1101/pdb.prot097352 Cold Spring Harb Protoc © 2019 Cold Spring Harbor Laboratory Press
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