Protocol

In Vitro Fertilization in Mice

  1. Naomi Nakagata

Abstract

The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β-cyclodextrin (MBCD) and fertilization medium with high calcium and reduced glutathione (GSH).

Footnotes

  • From the Manipulating the Mouse Embryo collection, edited by Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, and Andras Nagy.

This Article

  1. Published in Advance April 18, 2018, doi: 10.1101/pdb.prot094524 Cold Spring Harb Protoc © 2018 Cold Spring Harbor Laboratory Press
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  3. All Versions of this Article:
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