Protocol

Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems

  1. Anna Philpott3,4,5
  1. 1Future of Research, Abington, Massachusetts 02351
  2. 2Manylabs, San Francisco, California 94103
  3. 3Department of Oncology, MRC/Hutchison Research Centre, Cambridge CB21XZ, United Kingdom
  4. 4Wellcome Trust-Medical Research Centre Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB21QR, United Kingdom
  1. ↵5Correspondence: ap113{at}cam.ac.uk; garymcdow{at}gmail.com

Abstract

The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.

Footnotes

  • From the Xenopus collection, edited by Hazel L. Sive.

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This Article

  1. Published in Advance May 16, 2018, doi: 10.1101/pdb.prot103481 Cold Spring Harb Protoc © 2019 Cold Spring Harbor Laboratory Press
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  1. Profile for McDowell, G. S.http://orcid.org/0000-0002-9470-3799

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