Nucleic Acid Platform Technologies

Abstract

Every microarray experiment is based on a common format. First, a large number of nucleotide “spots” are arrayed onto a substrate, typically a glass slide, a silicon chip, or microbeads. Second, a complex population of nucleic acids (isolated from cells, selected from in vitro-synthesized libraries, or obtained from another source) is labeled, typically with fluorescent dyes. Third, the labeled nucleic acids are allowed to hybridize to their complementary spot(s) on the microarray. Fourth, the hybridized microarray is washed, allowing the amount of hybridized label to then be quantified. Analysis of the raw data generates a readout of the levels of each species of RNA in the original complex population. This introduction includes several examples of microarray applications and provides a discussion of the basic steps of most microarray experiments.