Transcriptomics and Proteomics Methods for Xenopus Embryos and Tissues
- 1The Francis Crick Institute, London NW1 1AT, United Kingdom
- 2Department of Molecular Developmental Biology, Radboud University, 6525 GA Nijmegen, The Netherlands
- 3Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, California 92697
- ↵4Correspondence: drmikegilchrist{at}gmail.com; g.veenstra{at}science.ru.nl; kwcho{at}uci.edu
Abstract
The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized RNA-based expression studies, while improvements in proteomics methods have enabled quantitative studies to attain better resolution. Here we introduce methods to undertake global analyses of gene expression through RNA and protein quantification in Xenopus embryos and tissues.
Footnotes
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From the Xenopus collection, edited by Hazel L. Sive.
