Raising Antibodies for Use in Xenopus

Maya Z. Piccinni; Matthew J. Guille

doi:10.1101/pdb.prot105585

Protocol

Raising Antibodies for Use in Xenopus

Maya Z. Piccinni and
Matthew J. Guille1

European Xenopus Resource Centre, Institute of Biological and Biomedical Sciences, University of Portsmouth, Portsmouth, Hampshire PO1 2DY, United Kingdom

↵1Correspondence: matthew.guille{at}port.ac.uk

Abstract

For work in Xenopus, frog-specific antibodies must usually be raised, although a few antibodies against mammalian proteins cross-react. To produce an immunogen for antibody production, human embryonic kidney (HEK) expression systems can be used as described here. For most laboratories, the actual method of raising the antibody is determined by local ethical regulations controlling the adjuvant and injection protocols used. Because these steps are often outsourced, they are not included in this protocol.

Footnotes

From the Xenopus collection, edited by Hazel L. Sive.

Articles citing this article

The Use of Xenopus for Cell Biology Applications Cold Spring Harb Protoc; 2021;
Confirming Antibody Specificity in Xenopus Cold Spring Harb Protoc; 2020;
Purifying Antibodies Raised against Xenopus Peptides Cold Spring Harb Protoc; 2020;
Assessing the Immune Response When Raising Antibodies for Use in Xenopus Cold Spring Harb Protoc; 2020;