Protocol

Bulk Dye Loading for In Vivo Calcium Imaging of Visual Responses in Populations of Xenopus Tectal Neurons

  1. Kurt Haas1
  1. Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia V6T2B5, Canada
  1. ↵1Correspondence: kurt.haas{at}ubc.ca

Abstract

Bulk loading of neurons with fluorescent calcium indicators in transparent albino Xenopus tadpoles offers a rapid and easy method for tracking sensory-evoked activity in large numbers of neurons within an awake developing brain circuit. In vivo two-photon time-lapse imaging of an image plane through the optic tectum allows defining receptive field properties from visual-evoked responses for studies of single-neuron and network-level encoding and plasticity. Here, we describe loading the Xenopus tadpole optic tectum with the membrane-permeable AM ester of Oregon Green 488 BAPTA-1 (OGB-1 AM) for in vivo imaging experiments.

Footnotes

  • From the Xenopus collection, edited by Hazel L. Sive.

  • Supplemental Material is available for this article at cshprotocols.cshlp.org.

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  1. Published in Advance March 29, 2021, doi: 10.1101/pdb.prot106831 Cold Spring Harb Protoc © 2022 Cold Spring Harbor Laboratory Press
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