Protocol

Modeling Human Genetic Disorders with CRISPR Technologies in Xenopus

  1. Robert M. Grainger3,4
  1. 1Department of Psychiatry and Behavioral Sciences, Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, California 94143, USA
  2. 2European Xenopus Resource Centre, School of Biological Sciences, University of Portsmouth, Portsmouth PO1 2UP, United Kingdom
  3. 3Department of Biology, University of Virginia, Charlottesville, Virginia 22904, USA
  1. ↵4Correspondence: helen.willsey{at}ucsf.edu; rmg9p{at}virginia.edu

Abstract

Combining the power of Xenopus developmental biology with CRISPR-based technologies promises great discoveries in understanding and treating human genetic disorders. Here we provide a practical pipeline for how to go from known disease gene(s) or risk gene(s) of interest to methods for gaining functional insight into the contribution of these genes to disorder etiology in humans.

Footnotes

  • From the Xenopus collection, edited by Hazel L. Sive.

This Article

  1. Published in Advance September 16, 2021, doi: 10.1101/pdb.prot106997 Cold Spring Harb Protoc © 2022 Cold Spring Harbor Laboratory Press
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  1. Profile for Willsey, H. R.http://orcid.org/0000-0001-8404-3291

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