Acoustic-Related Mating Behavior in Tethered and Free-Flying Mosquitoes

Abstract

Acoustics play an essential role in mosquito communication, particularly during courtship and mating. Mosquito mating occurs in flight and is coordinated by the perception of wingbeat tones. Flight tone frequencies have been shown to mediate sex recognition in Aedes, Anopheles, Culex, and Toxorhynchites genera and are thus a conserved feature of mating across the mosquito family (Culicidae). Upon recognizing a flying female, males respond phonotactically by lunging toward the female and initiating a precopulatory courtship flight interaction. During this interaction, males and females often harmonize their flight tones in a behavior known as harmonic convergence, and male acoustics display rapid frequency modulation. These acoustic phenomena have been characterized both in tethered and free-flying mosquitoes using similar audio recording and analysis methods. Further, the manipulation of mosquito acoustic-related mating behavior shows great promise as a tool for reproductive control strategies. In this brief methodological introduction, we provide an overview of the biological and technical concepts necessary for understanding the recording and analysis of mosquito mating acoustics.