EdU and Immunofluorescence Staining of Nothobranchius furzeri Organotypic Cultures
- 1Laboratory of Biology (BIO@SNS), Scuola Normale Superiore, 56126, Pisa, Italy
- 2Biology and Evolution of Marine Organisms Department (BEOM), Stazione Zoologica Anton Dohrn, 80121, Napoli, Italy
- ↵3Correspondence: alessandro.cellerino{at}sns.it
Abstract
Immunofluorescence is a widely used technique to visualize the localization of proteins of interest. Nucleoside analogs, such as 5-ethynyl-2′-deoxyuridine (EdU), are incorporated into newly synthesized DNA and enable permanent labeling of newly divided cells. Both these techniques can be applied to long-term organotypic culture of Nothobranchius furzeri in a fashion similar to that already described for tissue sections. We report here our optimized method for immunofluorescence and EdU staining of N. furzeri organotypic slices.
Footnotes
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From the African Turquoise Killifish collection, edited by Anne Brunet.
