Genetics and Gene Editing Methods in Xenopus laevis and Xenopus tropicalis
- 1European Xenopus Resource Centre, School of Biological Sciences, University of Portsmouth, Portsmouth PO1 2UP, United Kingdom
- 2Department of Biology, University of Virginia, Charlottesville, Virginia 22903, USA
- ↵3Correspondence: rmg9p{at}virginia.edu
Abstract
Our understanding of biological systems has for many years been heavily influenced by experimental approaches that exploit genetic methods. These include gain-of-function experiments that overexpress transgenes or ectopically express injected RNA and loss-of-function experiments that knock out genes or knock down RNAs. Here, we review how these methods have been applied in Xenopus frogs and introduce a variety of protocols for genetic manipulation of Xenopus laevis and Xenopus tropicalis.
Footnotes
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From the Xenopus collection, edited by Hazel L. Sive.
