Sequencing the Immunoglobulin Heavy-Chain Locus (IgH) in Turquoise Killifish (Nothobranchius furzeri)
- Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany; and Leibniz Institute on Aging, Fritz Lipmann Institute, 07745 Jena, Germany
- ↵1Correspondence: dvalenzano{at}leibniz-fli.de
Abstract
B cells undergo a process of somatic recombination leading to the synthesis of a staggering diversity of transmembrane and secreted antibodies. This process dates back to the evolution of jawed vertebrates and is pivotal to the origin of lymphocyte-based adaptive immune responses. Here, we optimized a sequencing-based protocol to characterize B-cell-specific IgH transcripts from bulk RNA in the African turquoise killifish, an emerging model organism characterized by naturally short life span and by a vast range of age-dependent dysfunctions.
Footnotes
-
From the African Turquoise Killifish collection, edited by Anne Brunet.
