Protocol

Breeding and Reproduction of the African Turquoise Killifish Nothobranchius furzeri

  1. Anne Brunet1,2,3
  1. 1Department of Genetics, Stanford University, Stanford, California 94305, USA
  2. 2Glenn Laboratories for the Biology of Aging, Stanford University, Stanford, California 94305, USA
  1. 3Correspondence: abrunet1{at}stanford.edu

Abstract

The successful breeding and reproduction of the African turquoise killifish Nothobranchius furzeri in a controlled laboratory setting are required to establish this fish species as a model system for studying vertebrate development and aging. Here, we describe a protocol to care for and hatch African turquoise killifish embryos, raise the juvenile fish to adulthood, and breed this species using sand as the breeding bedding. We also provide suggestions for generating a large quantity of good-quality embryos.

Footnotes

  • From the African Turquoise Killifish collection, edited by Anne Brunet.

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  1. Cold Spring Harb Protoc © 2023 Cold Spring Harbor Laboratory Press
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