Protocol

Generating Aedes aegypti Mutant Strains with Transgenic Cas9

  1. Omar S. Akbari1
  1. Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California 92093, USA
  1. ↵1Correspondence: oakbari{at}ucsd.edu

Abstract

Here, we provide a protocol for generating Aedes aegypti mutant strains via end-joining (EJ) or homology-directed repair (HDR) mechanisms using genetically encoded Cas9.

Footnotes

  • From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.

This Article

  1. Published in Advance March 17, 2023, doi: 10.1101/pdb.prot108085 Cold Spring Harb Protoc © 2023 Cold Spring Harbor Laboratory Press
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  1. Profile for Akbari, O. S.http://orcid.org/0000-0002-6853-9884

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