Protocol

Agar Plate-Based Method for the Selection of Antibiotic-Resistant Bacterial Strains

  1. Angelika Gründling2,3
  1. 1Microbiology, School of Biological and Chemical Sciences, National University of Galway, Galway H91 TK33, Ireland
  2. 2Section of Molecular Microbiology and Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, United Kingdom
  1. ↵3Correspondence: a.grundling{at}imperial.ac.uk

Abstract

Identifying the molecular mechanisms underlying antibiotic resistance is important, as it can reveal key information on the mode of action of a drug and provide insights for the development of novel or improved antimicrobials. Here, we describe an agar-based method for the selection of bacterial strains with increased antibiotic resistance, and how the increase in resistance can be confirmed by a spot-plating assay. As a specific example, we describe the selection of Staphylococcus aureus strains with increased resistance to oxacillin; however, the protocol can be easily adapted and used with other bacteria and antibiotics.

Footnotes

  • From the Experiments in Bacterial Genetics collection, edited by Lionello Bossi, Andrew Camilli, and Angelika Gründling.

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  • Preparation of Staphylococcus aureus Genomic DNA Using Promega Nuclei Lysis and Protein Precipitation Solutions, Followed by Additional Cleanup and Quantification Steps Cold Spring Harb Protoc; 2023;
  • Selection of Antibiotic-Resistant Bacterial Strains and Identification of Genomic Alterations by Whole-Genome Sequencing: Using Staphylococcus aureus and Oxacillin Resistance as an Example Cold Spring Harb Protoc; 2023;
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This Article

  1. Published in Advance April 28, 2023, doi: 10.1101/pdb.prot107897 Cold Spring Harb Protoc © 2023 Cold Spring Harbor Laboratory Press
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ORCID

  1. Profile for Gründling, A.http://orcid.org/0000-0002-6235-8687

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