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Protocol Identification of Editable Sites, Spacer Oligonucleotide Design, and Generation of the Gene-Targeting CRISPR–nCas9 Plasmid for Gene Disruption in Staphylococcus aureus Using the CRISPR–nCas9 and Cytidine Deaminase System

Angelika Gründling and
Quanjiang Ji

Cold Spring Harb Protoc; doi:10.1101/pdb.prot107924

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