Topic Introduction

Selection of Antibiotic-Resistant Bacterial Strains and Identification of Genomic Alterations by Whole-Genome Sequencing: Using Staphylococcus aureus and Oxacillin Resistance as an Example

  1. Angelika Gründling2,3
  1. 1Microbiology, School of Biological and Chemical Sciences, National University of Galway, Galway H91 TK33, Ireland
  2. 2Section of Molecular Microbiology and Medical Research Council Centre for Molecular Bacteriology and Infection, Imperial College London, London SW7 2AZ, United Kingdom
  1. ↵3Correspondence: a.grundling{at}imperial.ac.uk

Abstract

Here, we discuss methods for the selection of antibiotic-resistant bacteria and the use of high-throughput whole-genome sequencing for the identification of the underlying mutations. We comment on sample requirements and the choice of specific DNA preparation methods depending on the strain used and briefly introduce a workflow we use for the selection of Staphylococcus aureus strains with increased oxacillin resistance and identification of genomic alterations.

Footnotes

  • From the Experiments in Bacterial Genetics collection, edited by Lionello Bossi, Andrew Camilli, and Angelika Gründling.

This Article

  1. Published in Advance April 28, 2023, doi: 10.1101/pdb.top107896 Cold Spring Harb Protoc © 2023 Cold Spring Harbor Laboratory Press
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ORCID

  1. Profile for Gründling, A.http://orcid.org/0000-0002-6235-8687

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