Anopheles Adult Anesthesia, Feeding, and Sex Separation
- 1Entomology Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia 30333, USA
- 2CDC Foundation, Atlanta, Georgia 30308, USA
- ↵4Correspondence: mbenedict{at}cdc.gov
-
↵3 These authors contributed equally to this work.
Abstract
The adult stage is the only nonaquatic stage of the Anopheles mosquito. Both male and female Anopheles mosquitoes require access to a source of sugar to survive. In the insectary, a temperature of ∼27°C and 80% relative humidity and a cycle of 12 h light:12 h dark light, ideally with a sunrise and sunset period, are necessary minimum conditions to mimic their natural environment. Laboratory-reared Anopheles can survive for over a month; however, decreased activity and increased mortality may be observed ∼2 wk postemergence depending on the species and health of the colony. Details on how to maintain adults Anopheles are discussed here. Information and considerations on blood and sugar feeding are described. This protocol also provides instructions on how to differentiate male and female adult mosquitoes.
Footnotes
-
From the Mosquitoes collection, edited by Laura B. Duvall and Benjamin J. Matthews.
