Generating CRISPR Alleles in Drosophila
- 1Department of Neuroscience, Brown University, Providence, Rhode Island 02912, USA
- 2Carney Institute for Brain Science, Brown University, Providence, Rhode Island 02912, USA
- 3Pediatrics, Cell & Developmental Biology, University of California, San Diego, La Jolla, California 92093, USA
- 4Biological Sciences, Cell & Developmental Biology, University of California, San Diego, La Jolla, California 92093, USA
- ↵5Correspondence: oconnorgiles{at}brown.edu; jwildonger{at}ucsd.edu
Abstract
CRISPR gene editing is a versatile and efficient approach for generating a wide variety of genetic reagents in flies. This unparalleled ability to manipulate the genome has revolutionized neuroscience, allowing Drosophila neurobiologists to readily generate new alleles to probe gene function, investigate the functional consequences of disease-associated variants, tag endogenous proteins to follow their dynamic localization in neurons and glia, and much more. Here, we provide a comprehensive protocol for generating heritable mutations in Drosophila. We particularly focus on design considerations and tips for avoiding common errors to maximize the likelihood of successful gene editing.
Footnotes
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From the Drosophila Neurobiology collection, edited by Bing Zhang, Ellie Heckscher, Alex C. Keene, and Scott Waddell.
