Orientation of Implanted or Dissected Mouse Embryos for Histological Sectioning
- 1Department of Genetics and Development, Columbia University Medical Center, New York, New York 10032, USA
- 2Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA
- ↵3Correspondence: vep1{at}columbia.edu
Abstract
Histological analysis is an informative part of any mutational analysis. Interpretation of histological sections is much easier, and comparisons of mutant and wild-type embryos are more reliable if the plane of sectioning is precisely controlled. This protocol provides methods for obtaining sections with reproducible orientation for embryos between E4.5 and E9.5 still in the uterus and for dissected embryos E9.5 and older.
Footnotes
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From the Mouse Phenotypes collection by Virginia E. Papaioannou and Richard R. Behringer.
