Topic Introduction

Imaging Neuropeptide Release from Drosophila Clock and Motor Neurons

  1. Markus K. Klose
  1. Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
  1. ↵1Correspondence: elevitan{at}pitt.edu

Abstract

Electrophysiological studies of synaptic function do not robustly report release of neuropeptides and neurotrophins. These limitations have been overcome with the presynaptic expression of optical release reporters based on green fluorescent protein and fluorogen-activating protein. Here we describe how to image neuropeptide release in Drosophila at the neuromuscular junction and in the adult brain.

Footnotes

  • From the Drosophila Neurobiology collection, edited by Bing Zhang, Ellie Heckscher, Alex C. Keene, and Scott Waddell.

A more recent Protocol discussing this method is available

  • Topic Introduction Imaging the Drosophila Neuromuscular Junction (NMJ): Basic Optical Principles and Equipment
    • Edwin S. Levitan and
    • Dinara Shakiryanova
    Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.top92

This Article

  1. Published in Advance April 1, 2024, doi: 10.1101/pdb.top107798 Cold Spring Harb Protoc © 2024 Cold Spring Harbor Laboratory Press
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