Use of Maize (Zea mays L.) Mutator Transposon-Induced Mutants of the BonnMu Resource for Forward and Reverse Genetics Studies
- Yan Naing Win1,2,
- Martin Pöschel1,2,
- Tyll Stöcker3,
- Xuelian Du1,2,
- Alina Klaus1,
- Ben Wilhelm Braun1,2,
- Linnéa Lukas1,2,
- Alexa Brox1,2,
- Heiko Schoof3,
- Frank Hochholdinger1 and
- Caroline Marcon1,2,4
- 1Crop Functional Genomics, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, 53113 Bonn, Germany
- 2BonnMu: Reverse Genetic Resources, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, 53113 Bonn, Germany
- 3Crop Bioinformatics, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, 53115 Bonn, Germany
- ↵4Correspondence: marcon{at}uni-bonn.de
Abstract
The BonnMu resource represents a tagged collection of maize (Zea mays L.) Mutator (Mu) transposon-induced mutants, designed for functional genomics studies. Here, we describe the use of the BonnMu collection for identifying and characterizing mutations. Specifically, we describe workflows for use in both reverse and forward genetics strategies in maize. For reverse genetics, users first acquire a BonnMu F2 stock of interest based on data accessible at the Maize Genetics and Genomics Database (MaizeGDB). We provide details here for their subsequent propagation and for the confirmation of Mu insertions by genotyping via PCR, with the ultimate goal of establishing genotype–phenotype relationships of interest. For forward genetics studies, we describe a workflow that involves a combined approach of Mutant-Seq (Mu-Seq) and bulked segregant RNA-seq (BSR-Seq), to identify the causal gene underlying a mutant phenotype of interest.
Footnotes
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From the Maize collection, edited by Candice N. Hirsch and Marna D. Yandeau-Nelson. The entire Maize collection is available online at Cold Spring Harbor Protocols and can be accessed at https://cshprotocols.cshlp.org/.
