Early Release Articles

Last updated December 29, 2025

Early Release Articles are published online ahead of the issue in which they appear. The online first release date serves as the official date of publication. Upon release of the article's scheduled issue, the Early Release article (which may not be the final version) will move from this index to the issue's full Table of Contents in its final edited form.

January 27, 2026

    Protocol High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic E. coli
    • Adrienne N. Moran Lauter,
    • Anthony Assibi Mahama,
    • and M. Paul Scott
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108687
    Topic Introduction Grain Quality in Maize
    • Anthony Assibi Mahama,
    • Adrienne N. Moran Lauter,
    • and M. Paul Scott
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108476
    Protocol High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples
    • Adrienne N. Moran Lauter,
    • Anthony Assibi Mahama,
    • and M. Paul Scott
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108686

November 13, 2025

    Protocol Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource
    • Donald R. McCarty and
    • Karen E. Koch
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108688
    Topic Introduction The UniformMu National Public Resource: Transposon-Induced Mutant Seeds for Functional Genomics Studies in Maize
    • Karen E. Koch and
    • Donald R. McCarty
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108483

November 7, 2025

    Topic Introduction Insights from the Study of B-Cell Epitopes of a Microbial Pathogen by Phage Display
    • Gregg J. Silverman
    Cold Spring Harb Protoc; doi:10.1101/pdb.top107777
    Protocol Cloning and Selection from Antigen Fragment Libraries for Epitope Identification
    • Gregg J. Silverman
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108660

October 22, 2025

    Overview Advances in Phage Display—A Perspective
    • George P. Smith
    Cold Spring Harb Protoc; doi:10.1101/pdb.over107753

October 15, 2025

    Protocol Three-Point Bend Testing for Quantification of Maize Brace Roots Mechanics
    • Emilia Pierce,
    • Ashley N. Hostetler,
    • and Erin E. Sparks
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108668
    Topic Introduction Experimental Approaches for Assessing Root Mechanical Properties in Maize
    • Irene I. Ikiriko,
    • Ashley N. Hostetler,
    • and Erin E. Sparks
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108439
    Protocol In-Field Evaluation of Root Lodging Resistance of Whole Maize Plants
    • Jinzhong Yang,
    • Juan Liu,
    • Jinsheng Yang,
    • Xiaohu Wang,
    • and Yinchang Li
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108669

October 7, 2025

    Topic Introduction CRISPR–Cas-Directed Genome Editing in Maize
    • Bing Yang and
    • Kan Wang
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108448
    Protocol CRISPR–Cas9 Toolkit for Maize: Vector Design, Construction, and Analysis of Edited Plants
    • Si Nian Char,
    • Hua Liu,
    • James A. Birchler,
    • Kan Wang,
    • and Bing Yang
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108659

July 31, 2025

    Topic Introduction Anolis Lizards as a Model System for Studies of Gene Function in Reptile Development and Evolution
    • Christina E. Sabin,
    • James D. Lauderdale,
    • and Douglas B. Menke
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108535
    Protocol A Surgical Method for Oocyte Injection and CRISPR–Cas9 Mutagenesis in Anolis Lizards
    • Christina E. Sabin,
    • Sukhada P. Samudra,
    • Anna L. Iouchmanov,
    • Amber L. Rittgers,
    • James D. Lauderdale,
    • and Douglas B. Menke
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108652

July 16, 2025

    CORRIGENDUM Corrigendum: Inoculation of Maize Roots with Globisporangium ultimum var. ultimum to Study Pythium Root Rot
    • Harrison Hall,
    • Peyton Sorensen,
    • Tiffany Jamann,
    • and Santiago Mideros
    Cold Spring Harb Protoc; doi:10.1101/pdb.Corr108654
    Protocol Inoculation of Maize Roots with Globisporangium ultimum var. ultimum to Study Pythium Root Rot
    • Harrison Hall,
    • Peyton Sorensen,
    • Tiffany Jamann,
    • and Santiago Mideros
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108640

June 3, 2025

    Protocol Field Preparation and Planting Corn (Zea mays)
    • Melissa A. Draves,
    • Zachary B. Traylor,
    • Miriam Nancy Salazar-Vidal,
    • Sarah L. Fitzsimmons,
    • and Sherry Flint-Garcia
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108636
    Topic Introduction Pathogen Inoculation and Rating Strategies for Studying Maize Diseases
    • Peyton Sorensen,
    • Santiago Mideros,
    • and Tiffany Jamann
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108447
    Protocol How to Harvest and Store Corn (Zea mays)
    • Miriam Nancy Salazar-Vidal,
    • Melissa A. Draves,
    • Sarah L. Fitzsimmons,
    • Zachary B. Traylor,
    • and Sherry Flint-Garcia
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108639
    Topic Introduction Planting, Pollinating, Harvesting, and Monitoring Growth in Maize (Zea mays) for Research
    • Zachary B. Traylor,
    • Sarah L. Fitzsimmons,
    • Melissa A. Draves,
    • Miriam Nancy Salazar-Vidal,
    • William F. Tracy,
    • and Sherry Flint-Garcia
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108444
    Protocol How to Monitor Growth and Identify Developmental Stages of Maize (Zea mays)
    • Miriam Nancy Salazar-Vidal,
    • Melissa A. Draves,
    • Sarah L. Fitzsimmons,
    • Zachary B. Traylor,
    • William F. Tracy,
    • and Sherry Flint-Garcia
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108637
    Protocol Inoculation of Maize Leaves with the Bacterial Foliar Pathogen Clavibacter nebraskensis
    • Alexander Mullens,
    • Peyton Sorensen,
    • Julian Cooper,
    • and Tiffany Jamann
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108642
    Topic Introduction Uses and Opportunities for Ethyl Methanesulfonate Mutagenesis in Maize
    • Rajdeep S. Khangura,
    • Norman B. Best,
    • and Brian P. Dilkes
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108504
    Protocol High-Throughput Fluorescence Microscopy Using Aniline Blue Staining to Study the Maize–Exserohilum turcicum Pathosystem
    • Rashmi Pokhrel,
    • Alexander Mullens,
    • Peyton Sorensen,
    • Santiago Mideros,
    • and Tiffany Jamann
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108643
    Protocol Ethyl Methanesulfonate Treatment of Maize Seed for Recovery of Vegetative Mutant Sectors and Segregating Germinal Mutations
    • Rajdeep S. Khangura,
    • Norman B. Best,
    • and Brian P. Dilkes
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108650
    Protocol How to Pollinate Corn (Zea mays)
    • Sarah L. Fitzsimmons,
    • Miriam Nancy Salazar-Vidal,
    • Zachary B. Traylor,
    • Melissa A. Draves,
    • and Sherry Flint-Garcia
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108638
    Protocol Ethyl Methanesulfonate Treatment of Maize Pollen for Development of Segregating Mutant Populations or Targeted Mutagenesis
    • Rajdeep S. Khangura,
    • Norman B. Best,
    • and Brian P. Dilkes
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108651
    Protocol Inoculation of Maize Ears with Fusarium graminearum to Study Gibberella Ear Rot
    • Sarah Lipps,
    • Peyton Sorensen,
    • Mara Krone,
    • Santiago Mideros,
    • and Tiffany Jamann
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108641

April 17, 2025

    Protocol Sterol and Brassinosteroid Hormone Quantification by LC/MS of Picolinyl Ester Derivatives
    • Brian P. Dilkes and
    • Norman B. Best
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108646
    Protocol Analysis of Polar and Nonpolar Small Plant Growth Hormones and Quantification by LC/MS
    • Brian P. Dilkes and
    • Norman B. Best
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108647
    Topic Introduction Metabolite Profiling of Growth Regulatory Hormones from Maize Tissue
    • Brian P. Dilkes and
    • Norman B. Best
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108432

March 12, 2025

    Protocol Building AuxInYeast Synthetic Biology Strains for Biochemical Characterization of Maize Auxin Hormone Signaling Components
    • Román Ramos Báez,
    • Amy Lanctot,
    • and Britney L. Moss
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108634
    Protocol Testing AuxInYeast Synthetic Biology Strains via Fluorescence Flow Cytometry
    • Britney L. Moss,
    • Amy Lanctot,
    • and Román Ramos Báez
    Cold Spring Harb Protoc; doi:10.1101/pdb.prot108635
    Topic Introduction Synthetic Biology Approaches to Study Maize Signaling Pathways
    • Amy Lanctot,
    • Román Ramos Báez,
    • and Britney L. Moss
    Cold Spring Harb Protoc; doi:10.1101/pdb.top108450
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