Table of Contents

Protocols

• Epitope Mapping by Competition Assay

  • Ed Harlow and
  • David Lane

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4277
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• Epitope Mapping Using Synthetic Biotin-Labeled Peptides

  • Ed Harlow and
  • David Lane

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4278
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• Storage of Sera

  • Ed Harlow and
  • David Lane

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4279
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• Storage of Tissue Culture Supernatants

  • Ed Harlow and
  • David Lane

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4280
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• Storage of Ascitic Fluid

  • Ed Harlow and
  • David Lane

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4281
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• Labeling Antibodies with Fluorochromes

  • Ed Harlow and
  • David Lane

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4285
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• Labeling Antibodies with Biotin

  • Ed Harlow and
  • David Lane

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4286
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• Labeling Antibodies with Iodine

  • Ed Harlow and
  • David Lane

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4287
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• Labeling Monoclonal Antibodies by Biosynthesis

  • Ed Harlow and
  • David Lane

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4288
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• Visualizing Early Embryo Implantation Sites by Dye Injection

  • Sudhansu K. Dey

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4361
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• Vasectomy for Generation of Sterile Males: Access via Abdominal Wall

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4376
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• Vasectomy for Generation of Sterile Males: Access via Scrotal Sac

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4377
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• Oviduct Transfer

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4379
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• Uterine Transfer

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4380
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• Caesarean Section and Fostering

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4381
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• Transplantation of Tissues Under the Kidney Capsule

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4382
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• Subcutaneous Injection of Embryonic Stem (ES) Cells

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4383
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• Partial Hepatectomy

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4384
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• Splenectomy

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4385
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• Nephrectomy

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4386
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• Castration

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4387
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• Blood Collection by Tail Bleeding

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4388
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• Differentiating Embryonic Stem (ES) Cells into Embryoid Bodies

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4405
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• Culturing Trophoblast Stem (TS) Cell Lines

  • Janet Rossant

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4406
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• Derivation of Trophoblast Stem (TS) Cell Lines from Blastocysts

  • Janet Rossant

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4407
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• Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4409
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• Isolating Individual Embryonic Stem (ES) Cell Colonies by Picking

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4410
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• Freezing Embryonic Stem (ES) Cells in 96-Well Plates

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4411
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• Rapid Preparation of DNA from Cells in 96-Well Tissue Culture Dishes

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4413
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• Preparation of DNA from Cells in 24-Well Tissue Culture Plates

  • Andras Nagy,
  • Marina Gertsenstein,
  • Kristina Vintersten,
  • and Richard Behringer

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4414
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• Microscopic Analysis of Mitochondrial Transmembrane Potential (ΔΨ_m)

  • Shailaja Kasibhatla,
  • Gustavo P. Amarante-Mendes,
  • Deborah Finucane,
  • Thomas Brunner,
  • Ella Bossy-Wetzel,
  • and Douglas R. Green

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4462
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• Analysis of TUNEL Staining by Flow Cytometry to Detect Apoptosis

  • Shailaja Kasibhatla,
  • Gustavo P. Amarante-Mendes,
  • Deborah Finucane,
  • Thomas Brunner,
  • Ella Bossy-Wetzel,
  • and Douglas R. Green

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4463
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• Isolation of Fibroblasts from Mammalian Embryos

  • Anne Goldman

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4474
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• Isolation of Fibroblasts from Chicken Embryos

  • Anne Goldman

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4475
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• Lymphocyte Isolation and Culture

  • Gretchen J. Darlington

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4480
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• Epstein-Barr Virus Transformation of Lymphoblasts

  • Gretchen J. Darlington

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4481
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• Isolation and Freezing of Primary Mouse Embryonic Fibroblasts (MEF) For Feeder Plates

  • Linda C. Samuelson and
  • Joseph M. Metzger

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4482
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• Preparation of Mouse Embryonic Fibroblast (MEF) Feeder Plates

  • Linda C. Samuelson and
  • Joseph M. Metzger

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4483
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• Propagation of Pluripotent Mouse Embryonic Stem (ES) Cells

  • Linda C. Samuelson and
  • Joseph M. Metzger

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4484
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• Differentiation of Embryonic Stem (ES) Cells Using the Hanging Drop Method

  • Linda C. Samuelson and
  • Joseph M. Metzger

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4485
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• G₁/S Phase Synchronization using Double Thymidine Synchronization

  • Paul J. Galgano and
  • Carl L. Schildkraut

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4487
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• Cell Synchronization Using Centrifugal Elutriation

  • Paul J. Galgano and
  • Carl L. Schildkraut

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4490
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• Propidium Iodide (PI) Uptake Assay to Detect Apoptosis

  • Shailaja Kasibhatla,
  • Gustavo P. Amarante-Mendes,
  • Deborah Finucane,
  • Thomas Brunner,
  • Ella Bossy-Wetzel,
  • and Douglas R. Green

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4495
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• TUNEL Staining of Tissue Sections to Detect Apoptosis

  • Shailaja Kasibhatla,
  • Gustavo P. Amarante-Mendes,
  • Deborah Finucane,
  • Thomas Brunner,
  • Ella Bossy-Wetzel,
  • and Douglas R. Green

  Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4496
  • Full Text