Table of Contents

August 2006; 2006 (3)

Protocols

  • Growing Adherent Cells on Coverslips or Multiwell Slides

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4289

  • Growing Adherent Cells Directly on Tissue Dishes

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4290

  • Attaching Suspension Cells to Slides Using a Cytocentrifuge

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4291

  • Attaching Suspension Cells to Slides Using Chemical Linkers

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4292

  • Fixing Attached Cells in Organic Solvents

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4293

  • Fixing Attached Cells in Paraformaldehyde

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4294

  • Fixing Suspension Cells with Paraformaldehyde

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4295

  • Indirect Detection Using Horseradish Peroxidase-Labeled Reagents

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4296

  • Indirect Detection Using Fluorochrome-Labeled Reagents

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4297

  • Immunoblotting: Preparing Protein Solutions

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4298

  • Immunoblotting: Preparing Cell Lysates

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4299

  • Immunoblotting: Preparing Immunoprecipitated Proteins

    • Ed Harlow and
    • David Lane
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4300

  • Genotyping Embryonic Stem (ES) Cell Colonies Prior to Picking

    • Philippe Soriano
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4415

  • Preparation of Embryonic Stem (ES) Cells for Injection

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4416

  • Breaking the Tips of Embryonic Stem (ES) Cell Injection Needles

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4417

  • Injecting Blastocysts

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4418

  • Preparing Embryonic Stem (ES) Cells for Aggregation

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4419

  • Preparing the Aggregation Plate

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4420

  • Removal of Zona Pellucida

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4421

  • Production of Tetraploid Embryos

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4422

  • Assembling Aggregates between Diploid Embryos

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4423

  • Assembling Aggregates between Embryonic Stem (ES) Cells and Diploid Embryos

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4424

  • Assembling Aggregates between Diploid and Tetraploid Embryos

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4425

  • Assembling Aggregates between Embryonic Stem (ES) Cells and Tetraploid Embryos

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4426

  • Disaggregating Cleavage-Stage Embryos and the Inner Cell Mass (ICM) of Blastocysts into Individual Cells

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4427

  • Immunosurgery: Isolating the Inner Cell Mass (ICM) of Blastocysts

    • Andras Nagy,
    • Marina Gertsenstein,
    • Kristina Vintersten,
    • and Richard Behringer
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4428

  • Growth and Concentration of Chlorogonium for Culturing Freshwater Hypotrichs

    • Carolyn Jahn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4464

  • Flow Cytometric Analysis of Mitochondrial Transmembrane Potential (ΔΨm)

    • Shailaja Kasibhatla,
    • Gustavo P. Amarante-Mendes,
    • Deborah Finucane,
    • Thomas Brunner,
    • Ella Bossy-Wetzel,
    • and Douglas R. Green
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4465

  • Mass Culture of Freshwater Hypotrichs

    • Carolyn Jahn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4466

  • Culturing Marine Euplotids Using Dunaliella as a Food Source

    • Carolyn Jahn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4467

  • Refeeding Marine Euplotids with Bacteria

    • Carolyn Jahn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4468

  • Maintenance of Stocks of Marine Hypotrichs

    • Carolyn Jahn
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4469

  • Short-Term Storage of Tetrahymena Cultures

    • Melody T. Sweet and
    • C. David Allis
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4470

  • Long-Term Storage Storage of Unfrozen Tetrahymena Cultures in Maintenance Culture Medium

    • Melody T. Sweet and
    • C. David Allis
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4471

  • Long-Term Storage of Unfrozen Tetrahymena Cultures in Soybean Medium

    • Melody T. Sweet and
    • C. David Allis
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4472

  • Leukostat Staining of Cytospin Preparations to Detect Apoptosis

    • Shailaja Kasibhatla,
    • Gustavo P. Amarante-Mendes,
    • Deborah Finucane,
    • Thomas Brunner,
    • Ella Bossy-Wetzel,
    • and Douglas R. Green
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4491

  • Staining of Suspension Cells with Hoechst 33258 to Detect Apoptosis

    • Shailaja Kasibhatla,
    • Gustavo P. Amarante-Mendes,
    • Deborah Finucane,
    • Thomas Brunner,
    • Ella Bossy-Wetzel,
    • and Douglas R. Green
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4492

  • Acridine Orange/Ethidium Bromide (AO/EB) Staining to Detect Apoptosis

    • Shailaja Kasibhatla,
    • Gustavo P. Amarante-Mendes,
    • Deborah Finucane,
    • Thomas Brunner,
    • Ella Bossy-Wetzel,
    • and Douglas R. Green
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4493

  • Detection of Phosphatidylserine Externalization During Apoptosis

    • Shailaja Kasibhatla,
    • Gustavo P. Amarante-Mendes,
    • Deborah Finucane,
    • Thomas Brunner,
    • Ella Bossy-Wetzel,
    • and Douglas R. Green
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4494

  • Preparation of dsRNA Molecules for RNAi in Mouse Oocytes and Early Embryos

    • Paula Stein and
    • Petr Svoboda
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4508

  • Collection of Mouse Oocytes for RNAi

    • Paula Stein and
    • Petr Svoboda
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4509

  • Collection of Early Mouse Embryos for RNAi

    • Paula Stein and
    • Petr Svoboda
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4510

  • Microinjection of dsRNA into Mouse Oocytes and Early Embryos

    • Paula Stein and
    • Petr Svoboda
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4511

  • Synthesis of dsRNA for RNAi in Drosophila: Plasmid Template Method

    • Richard W. Carthew
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4512

  • RNA Isolation and RT-PCR from dsRNA-Treated Mouse Oocytes and Early Embryos

    • Paula Stein and
    • Petr Svoboda
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4513

  • Synthesis of dsRNA for RNAi in Drosophila: PCR Template Method

    • Richard W. Carthew
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4514

  • Synthesis of siRNA for RNAi in Drosophila

    • Richard W. Carthew
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4515

  • Microinjection of dsRNA into Drosophila Embryos

    • Richard W. Carthew
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4516

  • Delivery of dsRNA into Drosophila Embryos by Gene Gun

    • Richard W. Carthew
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4517

  • Semiquantitative RT-PCR for Phenotype Analysis of RNAi-Treated Drosophila Embryos

    • Richard W. Carthew
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4518

  • Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated Drosophila

    • Richard W. Carthew
    Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.prot4519
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